An introduction to molecular biology focusing on gene structure, organization, regulation and expression. Topics in genetic engineering and genome evolution are covered, as well as DNA replication, recombination, transcription and post-transcriptional mechanisms in both eukaryotic and prokaryotic cells.
- Describe genes, chromatin and chromosomes, with emphasis on genome sequences and gene numbers, as well as genome evolution.
- Explain DNA replication and recombination with emphasis on bacterial replication, and homologous and site-specific recombination.
- Discuss prokaryotic and eukaryotic transcription and translation while dwelling on RNA splicing and processing and RNA stability and localization.
- Differentiate between negative and positive regulation of a gene with emphasis on the lac operon in prokaryotes.
- Describe various RNA regulators such as MicroRNAs and RNA interference (RNAi).
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