CLS1205X Microbiology Didactic Lectures

Course Description

The Clinical Microbiology Didactic Lectures are designed to reinforce and strengthen the practical in-laboratory trainee experience. Areas in Clinical Microbiology that will covered are aerobic and anaerobic organisms, parasitology, antibiotic sensitivity testing and analysis of results, yeast, fungi, and Mycobacteriology.

Learning Outcomes

  • Successfully demonstrate the ability to introduce cells/organisms of various specimen types into a type of culture medium.
  • Successful demonstration and application of the Proper staining technique for the stains used in a typical clinical laboratory science laboratory (Wright's stain, Giemsa stain, Ziel-Neelsen Acid Fast stain, Kinyoun stain, Methylene Blue stain, Flagellar stain, Modified Acid-Fast stain, Trichrome stain, India Ink stain, and Lactophenol Cotton Blue stain).
  • An understanding of basic bacteriology including bacterial morphology, staining reaction, andgrowth requirements .
  • Evaluate primary culture plates and create atesting algorithm to identify possible pathogens .
  • Ability to perform and analyze antibiotic sensitivity testing .
  • Analyze antibiograms for trends of antibiotic resistance.
  • Ability to analyze and evaluate blood cultures with demonstration of biochemical testing algorithms in states of pathology.
  • Demonstrate the ability to correctly differentiate anaerobic pathogens from non-pathogens.
  • Demonstrate successful application of serological techniques with subsequent ability to successfully identify the pathogen(s).
  • Demonstration of proper specimen handling, use of a Biosafety cabinet, staining and correct biochemical test selection for organism identification using the acid fast culture technique.
  • Understanding of a fluorescence microscope's function, parts, staining techniques and competence in the operation of the microscope.
  • Ability to remember and apply laboratory safety policies and regulations.
  • Perform, analyze and evaluate if all necessary Quality Controls were attained.
  • Demonstration of successful inoculation of patient specimens onto appropriate mycology media for mycology testing.
  • Demonstrate correct and accurate identification of fungal isolates.
  • Employ appropriate culture techniques and biochemical testing for fungal identification.
  • Employ appropriate isolation techniques for viral pathogen(s), including tissue culture transfer and antibody testing algorithm sufficient for identification of the viral pathogen(s).