National University




BIO203A Introductory Microbiology Lab

Lead Faculty: Dr. Michael R. Maxwell

Course Description

This laboratory course introduced students to procedures for handling microbes, methods of identification of microorganisms (microscopic and by diagnostic media), preparation of stained slides and wet mounts, aseptic techniques, isolation of a single colony, preparation of a pure culture, inoculation and interpretation of select diagnostic tests. This two-month course is a combination of lecture and laboratory activities.

Learning Outcomes

  • Learn about laboratory safety, proper conduct, and correct handling of biological specimens.
  • Prepare, observe, and interpret stained slides of bacteria using microscopy.
  • Aseptically transfer, culture bacteria, and isolate individual colonies by using the streak technique.
  • Use differential and selective media to isolate and identify various bacteria.
  • Test microbial susceptibility using different chemical agents.
  • Culture bacteria from common surfaces and substrates.
  • Identify samples of unknown bacteria using deductions and systematic testing.

Prerequisite